Protein sample preparation and optimization

Towards biophysical characterization and integrative structural studies

Summary

This training school is aimed at structural biologists who want to improve their skills in sample analysis and optimization for structural techniques, in particular Cryo-EM. The course will cover different biochemical and biophysical techniques such as chromatography, dynamic and static light scattering, viscometry, circular dichroism, mass spectrometry, and differential scanning fluorimetry. Hands on training will be provided on basic quality control of protein as well as buffer and storage optimization. Trainees will be allowed to bring their own samples to use during the practical sessions. These sessions will be complemented with dry practical tutorials on purification strategies as well as on the choice of molecular scale biophysical measurements to complement structural determination. At the end of the course, trainees should have all the necessary know how to apply these measurements in their own institution.

 

Target audience

The course aims at trainees with little experience in the domain: early career staff, or people in need of new scientific skills: PhD students, post-Docs, research scientists, technical staff, core facility staff. 16 trainees will be selected for the full workshop and those who are not selected trainees will be offer the possibility to participate to the plenary lectures as free auditor

The plenary lectures will be open to free auditors after compulsory but free registration. 

Trainee Selection

This course will be open to 16 selected participants who should come from places where such training is not available locally or in close proximity.

Selection will be done on the basis of the downloadable trainee submission forms. The scientific committee will give priority to candidates who come from institution without expertise in this type of methodologies and technologies. The participants should show that the outcome of the course will be used rapidly in their projects and activities.

People willing to participate only as free auditors, will be selected on first arrive first serve basis.

 

Fee and Bursaries

Fees

  • Academic Fee: 
    • For full participants 75€, including coffee break, lunch and the workshop dinner.
    • For free auditors 0 € (Compulsary registration)
  • Indistrial Fee: Please contact us

The fee will be invoiced only after selction of the trainee by the scientific committee  

 

Bursaries:

  • 8  Full participants bursaries of 400€ will be offered
  • The bursaries will be given in priority to participants who have little or no access to institutional training budget such as PhD students and post-Docs.

 

Committee

Local Comittee

Bruno Baron, Sébastien Brûlé, Christine Detchepare, Patrick England, Sylviane Hoos, Bertrand Raynal 

 

Scientific 

Patrick England (Institut Pasteur, Paris, France)

Maria Garcia Alai (EMBL Hamburg Germany)

Stefan Knauer (Universität Bayreuth Germany)

Bertrand Raynal (Institut Pasteur, Paris, France)

Stephan Uebel (MaxPlank Institute of Biochemistry,  Munich Germany)

Michaela Wimmerová (CEITEC Brno Czech Republic)

 

   

Supported by

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COST action CA15126  

 

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Principal Sponsors

 

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with the participation of

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Flyer

Fyer

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